With the introduction of digital imaging. For many years researchers have been using darkrooms and exposing their blots to film to detect the chemiluminescent signal. Western blotting is used to detect the presence of a specific protein extracted from either cells or tissue. LI-COR provides the complete solution for. Kits include Immobilon ®-FL PVDF Membranes (0.45 μm, 10 cm x 10 cm), and either Intercept ™ (TBS) Blocking Buffer or Intercept (PBS) Blocking Buffer. Western blotting is a popular technique in cell and molecular biology. LI-COR Biosciences offers research solutions for robust Western blots and targeted therapeutics development.
The Western blotting kits provide enough secondary antibodies, blocking buffer, and membranes for 10 Western mini-blots.Įach kit contains one IRDye ® 800CW Dye-Labeled Secondary Antibody for the 800nm channel, plus an additional secondary antibody labeled with IRDye 680RD for the 700nm channel. Experiment with multiplex Western blotting for simultaneous two-color detection.Use near-infrared fluorescence detection on the Odyssey ® Imaging systems.Conveniently test LI-COR reagents and membranes for your Western blotting.These sample kits allow you to obtain a smaller quantity of infrared reagents and corresponding membranes to perform quantitative Western blots without committing to a large quantity of each. Western Blotting Kits can be used for quantitative Western blots or RNAi studies in which a phosphate-buffered saline (PBS) buffering system or a Tris-buffered saline (TBS) is used.Ĭhoose the Right Kit for You Convenient Kits for Multiplex Western Blots